Journal: Journal of Bacteriology
Article Title: Regulatory Role of Anti-Sigma Factor RsbW in Clostridioides difficile Stress Response, Persistence, and Infection
doi: 10.1128/jb.00466-22
Figure Lengend Snippet: Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro vertical diffusion chamber (VDC) infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.
Article Snippet: The Snapwell inserts, containing polarized Caco-2, HT29, and CCD 18co myofibroblasts, were each sandwiched between two compartments of the vertical diffusion chamber (VDC) (Harvard Apparatus, UK).
Techniques: Infection, In Vitro, Diffusion-based Assay, MANN-WHITNEY